Monday, June 30, 2008

What I Learned @ Bioassay Unit, HMRC, IMR







In Summary, these were the
activities that i had done during the past 2 and a half month of my industrial training.

  1. Information collection of Vernonia amygladina
  2. Preparation of mice to test the efficacy of a product & treatment on the next day
  3. Proper mouse handling
  4. Preparation of Buffer
    • Tris-Glycine SDS Electrophoresis Buffer (5X) (1Liter)
    • Transfer Buffer (10X) (1 Liter)
    • Tris-Buffered Saline (TBS)(10X) (1 Liter)
    • Phosphate-Buffered Saline (PBS) (10X)(1 Liter)
  5. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
    • Sample Loading
    • Dilution of 5X SDS Buffer to 1X
  6. Western Blot
    • Pre-Treatment of PVDF or Nitrocellulose Membrane
    • Dilution of 10X Transfer Buffer to 1X*
    • Preparation of 5% skim milk in 1X TBS
  7. Detection of Proteins, Immunologicallly
    • Preparation of 1X TBST
    • Protein washing
    • Dilution of Primary* and Secondary* Antibodies
    • Membrane incubation with antibody
    • Preparation of Enhance Chemiluminol Reagent
    • Film Exposure
    • Tracing and Stripping of membrane after exposure
  8. Cells Scrapping
  9. Membrane Staining Techniques
  10. DNA and RNA Quantification
  11. Total RNA digestion by DNase 1 – Amplification Grade
  12. Reverse Transcriptase polymerase Chain Reaction (RTPCR)
  13. Preparation of 1% and 2% Agarose Gel
    • Agarose Gel Electrophoresis
    • View result using UV Transluminator Device
  14. Post Mortem of mouse and rats (observation and minor help)
  15. Observation of the Acrylamide Gel preparation
  16. Histotechniques
  17. Protein Extraction
Wah, macam banyak kan? Hahaha! Korg sumer tertipu. Aku sajer jer kasik banyak2 biar nampak rajen sket. Hahahaha!

Aper2 pon, aku dah siapkan slide (actually it was for the presentation during lecturer visit). Boleh la korang donlod kat link bawah ni:-

To download, right click on link, choose "Open In New Window" and wait until your download link is processed by Mediafire File Host.

Link --> EIT - Abdul Aziz B. Ahmad

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